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Fig. 2. Phosphorimage cDNA expression profiles of osteoprogenitor/preosteoblast (OP/Pre-OB) colonies. OP/Pre-OB colonies were identified by the replica plating technique, and early and mature osteoblast colonies were identified morphologically by the cuboidal shape of osteoblasts and deposition of osteoid (OB) and mineralization of osteoid (Mature OB), respectively. The mRNA from each sample was reverse transcribed into cDNA and amplified by the poly(A)-PCR method. The resulting cDNA amplified from each colony was then probed for the expression of total cDNA (a control for the amplification procedure), the various bone-related proteins (COLL-I, ALP, OPN, BSP and OCN, PTH1R, PTHrP), and cytokine receptor messages (FGF-R1 and PDGF-R{alpha}) as specified. Each vertical lane is the cDNA from the same colony. The exposure is the same for all colonies for each probe. These colonies are in random order within each category.