Fig. 3. Peroxisomal import is defective in prx-5(RNAi) nematodes. Ex[p_HSP16/2GFP-SKL] nematodes were injected with dsRNA targeted to exon 6 of prx-5. Progeny were heat-shocked for 2 hours at 30°C and recovered for 1 hour. A disruption of peroxisomal localization of the GFP—SKL is seen in (A), which results in the GFP remaining cytosolic when compared with a control nematode in (B). In some animals we observed a mosaic of GFP subcellular localization, in that both cells with cytosolic and punctate GFP were observed in the same animal (C). Cells with GFP in all panels are intestinal cells. Bars, 5 µm.