Fig. 7. Effect of chemical glycolysis inhibition on type-II collagen expression. Wild-type epiphyseal chondrocytes were incubated with or without 0.2 M 2-deoxyglucose, cultured under normoxic or hypoxic conditions for 22 hours, and type-II collagen gene expression was analysed by quantitative real-time PCR. Bars represent -fold changes of mRNA levels compared with untreated values (normoxic expression taken as 1.0). Data are given as mean ± standard deviations (triplicates from three independent experiments). **P0.01.