Fig. 5. Developmental potential of BMSSCs in vitro. Primary cultures of cells derived from STRO-1^BRIGHT/VCAM-1⁺ marrow cells were induced under osteogenic, adipocytic or chondrocytic conditions. (A) Mineralized deposits stained positively with the von Kossa reagent (arrow) formed within 4 weeks of culture under osteoinductive conditions (200x). (B) The presence of clusters of lipid containing adipocytes were also detected by Oil red-O staining (arrow) within 2 weeks of adipogenic induction (200x). (C) In aggregate cultures, collagen type II was present throughout the cellular mass following 3 weeks of chondrogenic induction (arrow) (200x).