Fig. 6. H-7 and staurosporine enhance the PT-mediated permeabilization of BCECs. BCEC monolayers were exposed to medium, medium containing the drug H-7 (50 µM), staurosporine (50 nM), or PT (200 ng/ml) alone or medium with H-7 in combination with PT for 4 hours (+1 hour for the permeability assay). The transendothelial HRP transport was assessed by measuring HRP concentration spectrophotometrically in the lower compartment. OD values were representative of the traversed HRP as a measure of relative permeability. The standard deviations (error bars, n=6) are indicated. (A) Time-course of relative permeability upon incubation with PT and PKC inhibitors H-7 or staurosporine. (B) Effect of PKC inhibitors H-7 and staurosporine on the PT induced permeability after 4 hours of incubation.