Fig. 7. PKC-activating cellular drugs dioctanoyl-sn-glycerol and PMA reduce the PT-mediated permeabilization of endothelial monolayers. BCEC monolayers were exposed to medium, medium containing the drugs dioctanoyl-sn-glycerol (100 µg/ml) or PMA (500 nM), alone or in combination with PT (200 ng/ml) for 4 hours (+1 hour for the permeability assay). The transendothelial HRP transport was assessed by measuring HRP concentration spectrophotometrically in the lower compartment. OD values indicated the traversed HRP as a measure of relative permeability. The standard deviations (error bars, n=6) are indicated.