Fig. 8. Effect of the PI3 kinase inhibitors wortmannin and LY294002 on PT-mediated permeabilization of BCEC monolayers. BCEC monolayers were exposed to medium, medium containing wortmannin (100 nM), LY294002 (10 µM) or PT (200 ng/ml) alone or medium with wortmannin or LY294002 in combination with PT for 4 hours (+1 hour for the permeability assay). The transendothelial HRP transport was assessed by measuring HRP concentration spectrophotometrically in the lower compartment. OD values indicated the traversed HRP as a measure of relative permeability. The standard deviations (error bars, n=6) are indicated.