Fig. 6. Co-immunoprecipitation of TBP and TAF7L from testis extracts. (A) Immunoblots detecting TBP, TAF7 and TAF7L in cytoplasmic extract (CE) and nuclear extract (NE). (B) Immunoprecipitation of TBP from cytoplasmic extract. SN is the immunoprecipitate supernatant; IP the immunoprecipitated peptide-eluted fraction. (C) Immunoprecipitation of TBP from nuclear extract. (D) Detection of the presence of TBP and TAFs in total extracts from elutriation purified pachytene spermatocytes and haploid cells. The blot was first probed with antibodies against TBP, TAF7 and TAF7L and then reprobed with antibody against ACT and subsequently with TAF4. (E-F) The spermatocyte and haploid cell extracts (E) were immunoprecipitated with anti-TBP antibody. SN and IP are as described above. A small amount of IgG heavy chain which has leaked from the resin is visible in panel F, lane 3. The immunoblots were first probed with antibody against TBP, TAF7 and TAF7L (upper panel) and then reprobed with anti-TAF6 (lower panel). The presence of non-specific signal in lower panel F is indicated by asterisks (*).