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Fig. 1. DLin-7 encodes a single-PDZ domain protein. (A) Exon-intron structure of the DLin-7/CG7662 locus (black boxes, ORF) from the cytological interval 96B19 (genomic scaffold AE003750). (B) RT-PCR performed on poly(A)+ RNA of different developmental stages (staged embryos in hours, L1-L3, larval stages; pP, prepupa; P, pupa; F, female adult). (C) Northern blot of embryonic poly(A)+ RNA hybridized with a probe covering the L27 and PDZ domain of DLin-7. (D) Protein structure of DLin-7 and two putative orthologues from mammals (MALS3) and C. elegans (LIN-7). The green and lilac colours indicate the L27 and the PDZ domains, respectively. The percentages of amino acid identities of the two domains with respect to the corresponding domains of DLin-7 are shown. (E) Western blot analysis of wild-type embryos (1) and embryos overexpressing a Flag-tagged DLin-7 transgene (2) probed with anti-DLin-7 antibodies. Note the additional, slightly larger band in lane 2 that corresponds to the Flag-DLin-7 protein.