Fig. 5. DLin-7 is expressed in the CNS and at NMJs. (A) Ventral nerve cord of a 3rd instar larva. DLin-7 is enriched in the neuropil area. Arrows mark the periphery of the cortical area. (B-D). Muscle 12 at abdominal segment A3 is innervated by four different motornerve terminals (Ib, Is, II, III) as revealed by anti-HRP immunoreactivity (B), but only type I boutons exhibit clear DLin-7 specific immunoreactivity (C,D). (E-G) Confocal section of a branch of the NMJ at muscle 6. Note that the presynaptic marker HRP (E) is largely surrounded by DLin-7 specific immunoreactivity (F,G). (H-J) Confocal section of a NMJ at muscle 12, double-labeled with anti-Dlg (I) and anti-DLin-7 (J) antibodies. Arrowhead marks a site of reduced immunoreactivity. Bar, 20 µm (A); 10 µm (B-J).