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Fig. 7. Dlg-dependent localization of transgenically expressed Flag-DLin-7 at NMJs. (A) Western blot analysis on body wall muscle extracts using anti-DLin-7. A Coomassie staining was used as a loading control to ensure that equal amounts of body wall extracts were analyzed. M, molecular weight marker; lane 1, w1118; lane 2, dlg+;;UAS-Flag-DLin-7/Gal4-C57; lane 3, dlgXI-2/Y;;UAS-Flag-DLin-7/Gal4-C57. (B-G) Confocal sections of synaptic boutons at muscle 12 double-labeled with anti-HRP (B,D,F) and anti-Flag (C,E,G). (B,C) w1118; (D,E) dlg+;;UAS-Flag-DLin-7/Gal4-C57; (F,G) dlgXI-2/Y;;UAS-Flag-DLin-7/Gal4-C57.