Fig. 9. Ectopic expression of EGFP-Dlg-S97 and Dlg-S97N-EGFP. (A-I) Confocal sections of wing imaginal disc epithelia expressing EGFP-Dlg-S97 (A-C), EGFP-Dlg-S97 and Flag-DLin-7 (D-F) or Dlg-S97N-EGFP (G-I) under the control of ptc-Gal4, co-stained with anti-DLin-7 antibodies (B,E,H). Areas flanking the ptc-expression domain served as an internal control to assess possible effects on the subcellular distribution of DLin-7. Merged images are shown in C, F and I. No striking redistribution of DLin-7 was caused by either EGFP-Dlg-S97, which was enriched basolaterally (depicted by arrowheads in C,F), or Dlg-S97N-EGFP, which exhibited strong nuclear localization (G). (J-L) Mislocalization of DLin-7 in nuclei of muscles 6 and 7 upon Gal4-C57 driven expression of Dlg-S97N-EGFP. Each image represents a stack of 20 optical sections taken at 0.5 µm steps. Nuclear enrichment of Dlg-S97N-EGFP (G) was paralleled by partial targeting of DLin-7 to nuclei (H), which was not observed in w1118 (I). Arrows mark selected nuclei. Bars, 10 µm (in I for panels A-I; in L, for panels J-L).