Fig. 2. Time course of the effect of 25 mM glucose on lipogenic enzymes in myotubes. After 48 hours in serum-free medium containing 5 mM glucose, contracting myotubes were incubated for the indicated times with 25 mM glucose. Total protein content was then extracted, separated by SDS-PAGE and analysed for ACC2, FAS, HKII and CPT2 protein levels, and for the phosphorylation state of ACC protein (A). (B) ACC activity, deduced from the ratio between ACC2 and phosphorylated ACC protein level. Western blots represent three independent experiments.