Fig. 5. Silencing of SREBP-1 protein in contracting myotubes. After 48 hours in serum-free medium containing 5 mM glucose, myotubes were transfected with a SREBP-1 siRNA duplex. After 48 hours, total protein extracts were prepared at 0 hours, 1 hour, 2 hours or 3 hours after the addition of 25 mM glucose (G25). Western blotting was performed to detect SREBP-1c precursor (p), FAS, ACC2 and CPT2 proteins. Representative western blots of three independent experiments are shown.