Fig. 1. -PKC western blot standard curve. Purified -PKC was subjected to SDS-PAGE and immunoblotted as described in Materials and Methods. (A) Bands were visualized by ECL and quantified by densitometry. (B) Integrated optical densities (OD) were plotted as a function of -PKC mass. To ensure linearity of -PKC binding in all subsequent experiments, only OD values below 13 were used for quantification.