Fig. 3. Phase contrast (A) and fluorescent (B) images of myofilament associated
-PKC.
-PKC is activated with 50 µM arachidonic acid (AA) and the activated
-PKC is associated with myofilaments near Z-lines as indicated by arrows in the overlay image (C). A confocal image of a skinned myocyte decorated with
-PKC, an anti-
-PKC primary antibody and Alexa 488 secondary antibody (D) Magnification x1000.