Fig. 4. Isoform specificity of PKC binding to cardiac myofibrils. (A) Western blots of myofibril pellets after incubation with
-PKC,
-PKC or
-PKC. Below each lane is indicated the PKC activators used to stimulate binding. na, No activators. AA, 50 µM arachidonic acid. AA + DAG, 50 µM AA plus 25 µM dioctanoylglycerol.
M is
-PKC gel/blot marker, and
M is
-PKC gel/blot marker.