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Fig. 4. Isoform specificity of PKC binding to cardiac myofibrils. (A) Western blots of myofibril pellets after incubation with {alpha}-PKC, {delta}-PKC or {epsilon}-PKC. Below each lane is indicated the PKC activators used to stimulate binding. na, No activators. AA, 50 µM arachidonic acid. AA + DAG, 50 µM AA plus 25 µM dioctanoylglycerol. {epsilon}M is {epsilon}-PKC gel/blot marker, and {delta}M is {delta}-PKC gel/blot marker.