Fig. 6. Properties of -PKC binding to isolated F-actin. (A) Summary of -PKC binding to F-actin in the absence and presence of 50 µM arachidonic acid (AA). The line with solid circles (in the presence of AA) represents a fit to: B/B_max=[-PKC]/([-PKC] + EC₅₀), with EC₅₀=110 nM and B_max=18 pmol (or 180 pmol/mg protein). The stoichiometry of binding was 18 pmols -PKC bound per 2 nmoles actin monomers or a 1/110 ratio. Open circles represent -PKC with F-actin in the absence of AA. (B) Summary of inhibition of -PKC binding to F-actin by synthetic peptides. Data is represented as mean±s.e.m. from five experiments.