Fig. 1. Palmitoylated wt GAD65-GFP colocalizes with wt HA-Rab5a in Golgi membranes and in axon termini but not in somatodendritic puncta. (A) Confocal images of a hippocampal neuron cotransfected with plasmids encoding wt GAD65-GFP and HA-tagged wt Rab5a. Neurons were double immunolabeled for GFP and HA. GAD65-GFP colocalizes with wt Rab5a in axonal puncta (arrowheads, enlarged frame a) and in Golgi membranes (enlarged frame b) but not in dendritic puncta (enlarged frame c). Scale bars, 10 µm. (B) The percentage of HA-Rab5a(wt)-positive puncta expressing wt GAD65-GFP was analyzed as described in Materials and Methods. The yellow color indicates the percentage of HA-Rab5a(wt)-positive puncta expressing wt GAD65-GFP.