Fig. 3. EEA1-positive somatodendritic early endosomes are devoid of GAD65. Confocal images of hippocampal neurons expressing either GFP-tagged wt Rab5a or GAD65-GFP and immunolabeled for GFP and endogenous EEA1. (A) Wt Rab5a colocalizes with EEA1 in early endosomes in the soma and proximal dendrites (enlarged frame). (B) Enlarged view of a distal axon (arrowheads) and distal dendrites (arrows) of the neuron shown in A, reveals colocalization of Rab5a and EEA1 in dendritic puncta and lack of EEA1 in axonal puncta containing Rab5a. (C) GAD65 is absent from somatodendritic early endosomes containing EEA1. (D) EEA1 is absent from presynaptic clusters (arrowheads) containing GAD65-GFP. Scale bars, 10 µm.