(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 9. The Rab5a(S34N) dominant negative mutant inhibits axonal trafficking and presynaptic clustering of VGAT. Confocal images of hippocampal neurons transfected with plasmids encoding VGAT-HA and GFP-tagged Rab5a(S34N). Neurons were double immunolabeled for HA and GFP. (A) A view of the soma reveals that VGAT-HA colocalizes with GFP-Rab5a(S34N) in the Golgi compartment. (B) A view of a neuron expressing VGAT-HA and GFP-Rab5a(S34N). The red color was enhanced to show the axon. VGAT shows a weak diffuse staining in axons and is not detected in presynaptic clusters in the proximal (enlarged frame a) or in the distal axon (enlarged frame b). Scale bars, 10 µm.