Fig. 3. The dLBR is localized to the inner nuclear membrane. Indirect immunofluorescence microscopy (A-C; A'-C') of S2 cells after staining with antibodies against the dLBR (A,A'), lamin Dm0 (B,B') and -tubulin (C,C'). Cells were fixed with formaldehyde and then permeabilized with Triton X-100 (A-C) or digitonin (A'-C'). The inner nuclear membrane and nuclear interior are only accessible to antibodies in Triton-treated cells. Scale bars, 10 µm.