Fig. 1. (A) Western blot analysis of nuclear hormone receptor expression in freshly isolated human urothelium and cultured NHU cell lines. Protein lysates (40 µg/lane) extracted from human urothelium and from three independent NHU cell lines were loaded onto a 10% SDS-polyacrylamide gel, electrophoresed and transferred to nitrocellulose membrane. Membranes were probed with specific antibodies against PPAR and RXR. (B) Phase contrast morphology of NHU cells grown to 70% confluence and incubated in the absence or presence of TZ (1 µM or 100 µM, as indicated) for 48 hours. Bar, 100 µm.