Fig. 1. Features of Arabidopsis C-adaptin constructs. (A) At-C-Ad compared with its mammalian ortholog. GST-fusion proteins of At-C-Ad comprising either the full ear region (residues 536-1013; GST-At-C-h-ear) or its truncated version (residues 536-759; GST-At-C-h-ear). (B) Three-dimensional structure modelling of the hinge ear region of At-C-Ad was visualized using the Rasmol program, version 2.6. (C) Distribution of the specific binding sites within the two subdomains of mouse and Arabidopsis C-adaptin ear regions. (D) Alignment of the three C-ear binding-sites. Amino acid exchanges are underlined. Note the high degree of identity. (E) Coomassie-stained gels of the purified GST-fusion protein constructs of the At-C-Ad hinge ear region. (1) GST-At-C-h-ear, (2) GST-At-C-h-ear marked by a star and (3) control GST.