Fig. 5. Binding of At-C-Ad and At-AP180 measured by plasmon resonance (BIAcore 3000). The full-length ear construct and the truncated version of At-C-Ad were immobilized on CM-5 chips and H₆-At-AP180ENTH was passed over the sensor surface for 1 minute (association) followed by a 1 minute wash with running buffer (dissociation). (A) The full ear construct binds H₆-At-AP180ENTH with high affinity (k_D=35 nM) compared with the truncated version At-C-hear (k_D=682 nM). (B) The binding between GST-At-C-h-ear and H₆-At-AP180ENTH is concentration dependent as shown by increasing amounts of the At-AP180 construct.