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Fig. 8. H6-At-AP180{delta}ENTH assembles clathrin triskelia into cages of regular size. Electron micrographs (negative staining) of the reassembly experiment shown in Fig. 7. Assembly activity of clathrin alone (A), clathrin reassembled in a threefold excess of H6-At-AP180{delta}ENTH (B), and clathrin reassembled in a threefold excess of GST-At-AP180{delta}ENTH-DLL/AAA (C). Close-ups of the cages are inserted. Bar, 250 nm in the overviews and 50 nm in the close-ups. (D) Size distribution of assembled cages from (B) is quantified in bar charts. Note the narrow size distribution.