Fig. 6. Anisomycin introduced redistribution of Cx43 and gap-junction plaques. Cx43 was visualized as green spots by indirect immunofluorescence using FITC-labelled secondary antibody. Two sets of quadruple photographs are shown containing immunofluorescence images (IF) and Nomarski differential interference contrast images (DIC) of the same fields. These are typical images of each treatment group. Each treatment group was exposed to 0.02% dimethyl sulfoxide as vehicle alone for 90 minutes (control), anisomycin at 10 µg ml^-1 for 60 minutes, SB203580 plus anisomycin (pretreatment of SB203580 for 30 minutes, then co-treatment with anisomycin for an additional 60 minutes) or SB203580 alone for 90 minutes. Cytoplasmic staining for Cx43 was also observed (indicated by white arrows). All images in each panel are of the same magnification. Scale bar, 20 µm.