Fig. 7. Western blot analysis of Cx43 protein expression in cells treated with anisomycin and/or SB203580. Each treatment group was exposed to 0.02% dimethyl sulfoxide as vehicle alone for 90 minutes (control), anisomycin at 10 µg ml^-1 for 60 minutes, SB203580 plus anisomycin (pretreatment of SB203580 for 30 minutes, then co-treatment with anisomycin for an additional 60 minutes) or SB203580 alone for 90 minutes. (A) A typical western blot. Whole-cell extracts (20 µg per lane) were probed with an antibody against Cx43 or against occludin, ß-catenin as reference proteins, and against ß-actin as a control for protein loading. (B) The relative band intensities of P1, P2 and P0 of Cx43. (C) Autoradiograph of [³²P]-orthophosphate labelled Cx43 and its densitometric analysis on an SDS-PAGE gel. Results are from one representative experiment of two separate experiments. To assess each sample, the total value of control radiation was arbitrarily set at 1.