Fig. 5. Nuage clusters and particles in mutants. (A,C,E) Immunostaining of VasGFP. (B,D,F-H) Immunostaining of GFPAub. In wild-type nurse cells the pattern of VasGFP (A) and GFPAub (B) includes perinuclear nuage clusters (arrows) and cytoplasmic particles (arrowheads). Perinuclear nuage appears smoother in aub^HN2/aub^QC42 than in wild type, but nuage particles are unaffected (C). Very few nuage clusters and particles remain in vas^AS/vas^AS nurse cells (D). In the spnE^E616/spnE³⁹⁸⁷ mutant nuage particles are lost and the perinuclear nuage is smoother (E). Nuage clusters and particles appear wild type in tudor¹-mutant nurse cells (F). Nuage in wild-type spermatocytes is readily detectable using GFPAub (G), and is completely disrupted in vas^AS males (H). Scale bars, 1 µm (G,H), 5 µm (A,D), 10 µm (B,C,E,F).