Fig. 5. Mannose capped mycobacterial glycolipid inhibits lysosomal delivery to phagosomes. F-DXT-loaded THP-1 cells were stained with PKH and allowed to ingest latex beads in the presence of BSA (control) or various concentrations of soluble cell wall fraction (CW), LAM or PIM. Phagosomes were prepared at 2 hours post phagocytosis and analyzed by flow cytometry as described in Fig. 3. The data shown are means±s.d. of three separate experiments. The value on the top of each bar represent the proportion of phagosomes fusing with lysosomes.