Fig. 1. LSP1 interacts with ERK/MAP kinase pathway components. (A) W10 cells were lysed in 50 mM Tris pH 7.5, 5 mM EDTA, 0.5% CHAPS, and PKCßI, MEK1 and ERK2 were immunoprecipitated and analyzed for the presence of LSP1 by western blotting. No antibodies were added to control assays (cntrl). (B) Pull down experiments were performed using GST, GST-ERK2 or GST-MEK1 recombinant proteins and CHAPS-soluble W10 cell lysates and analyzed for the presence of LSP1. (C) To check recovery of GST and GST fusion proteins the same western blot shown in B was reprobed with an anti-GST antibody. All samples were analyzed on one gel but only the relevant portion of each lane containing the recovered GST or GST fusion protein is shown.