Fig. 3. LSP1 targets KSR, MEK1 and ERK2 to peripheral actin filaments. The LSP1^neg BW5147 cells (upper panels) and the LSP1⁺ transfectant T22 (lower panels) were stained for LSP1, KSR, MEK1, ERK2 and F-actin. Merged images show that LSP1, KSR, MEK1 or ERK2 (red) co-localize with F-actin (green) in the presence of LSP1 (lower panels) but not in the absence of LSP1 (upper panels). Some randomly chosen areas of co-localization indicated by a yellow color are identified by arrows. The insets show staining for LSP1 (blue). Scale bar: 10 µm.