Fig. 5. SLMAP3M1 sequences target GFP to centrosomes. (A) GFP-SLMAPM1 fusion protein used for transient transfection studies. Depicted are the GFP tag, the FHA domain, PEST motif, leucine zippers and the transmembrane (TM) domain. (B) Localization of GFP-SLMAP fusion proteins transiently expressed in NIH 3T3 cells was visualized by live microscopy. GFP fluorescence was detected at centrosomes in mitotic cells expressing GFP-SLMAP3M1 (a, two closely spaced dots). No green fluorescence was observed at centrosomes in control mitotic cells transfected with GFP-pcDNA alone (c). Co-incidence of the -tubulin signal (d) and GFP fluorescence (b) occurred in paraformaldehyde fixed mitotic cells expressing GFP-SLMAP3M1. Bar, 25 µm.