Fig. 7. SLMAP overexpression affects cell proliferation. (A) Overexpression of GFP-tagged SLMAPs inhibits BrdU uptake in NIH 3T3 cells. NIH 3T3 cells were transfected with either GFPpcDNA3 (green bar), GFP-SLMAP3M1 (red bar), GFP-SLMAP3M2 (yellow bar) or GFP-SLMAP3M2C (blue bar). Thirty-six hours post-transfection, cells were labeled with BrdU (100 mmol/l) for 2, 8, 12 or 24 hours. Ethanol fixed cells were co-stained with anti-mouse BrdU antibody and anti-rabbit GFP antibody. Transfected cells were identified by their green fluorescence and the number of cells with co-existing nuclear red fluorescence (BrdU positive cells) was determined. The mean results of at least three independent experiments for each labeling period are shown. For each transfectant and condition, a minimum of 200 cells were scored. Error bars represent standard deviations. (B) FACS profiles of GFP-pcDNA3 (i) and GFP-SLMAP3M2 (ii) transfected NIH 3T3 cells. At 48 hours post-transfection, cells were incubated with propidium iodide to stain DNA (Pestov et al., 1999).