Fig. 7. The delay of spindle elongation in scn1-17 was not dependent on the spindle checkpoint. (A) The double mutant scn1-17 mad2 cells were cultured at 20°C for 9 hours in the YPD medium and cells were fixed for staining by antibodies against tubulin (red) and Sa d1 (green). DAPI (blue) staining was done for DNA. The late anaphase cells were designated as A. Late anaphase cells were observed in scn1 mad2 as frequent as in the scn1 single mutant cells. Bar, 10 µm. (B) The pole-to-pole distance in mitotic cells was measured in the wild-type, scn1 and scn1 mad2 cells that were cultured at 20°C for 9 hours in YPD. Cells were stained with anti-Sad1 (SPB) and anti-TAT1 (tubulin). Over 100 mitotic cells of each strain were analyzed. The filled arrowheads indicate the size of the wild-type spindle at metaphase. The solid bar indicates the sizes of anaphase spindle that were abundantly seen in scn1 and scn1 mad2 cells. The cells showing anaphase spindle were accumulated in scn1 mad2 as much as in scn1 single mutant cells.