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Fig. 1. Strains lacking ARL1 are sensitive to hygromycin B and TMA. The parental strains PSY316 (ARL1; left) and MA03 (arl1; right), were struck onto YPAD medium (Adams et al., 1997; Sherman et al., 1974) containing no additions (left), 0.1 mg ml–1 hygromycin B (middle) or 0.25 M TMA (right), without other additions (top) or with the addition of 100 mM KCl (bottom). The plates were incubated for 3 days at 30°C and photographed.