Fig. 4. Mutants deleted for genes obtained in the high-copy-number suppressor screen are variously sensitive to hygromycin B. Selected homozygous diploid deletion strains (from the Deletion Collection) were grown overnight in YPAD liquid medium to saturation. Serial tenfold dilutions in water were performed in a multiwell plate and then spotted onto solid medium with a replicator tool. Strains were tested on YPAD medium with or without 0.1 mg ml^–1 hygromycin B and with or without 100 mM KCl. Cells grown in YPAD + KCl grew as well as cells on YPAD alone and are therefore not shown. Cells were grown for 4 days at 30°C then photographed.