Fig. 9. Cytotoxicity of Shiga toxin in HeLa and BHK cells. HeLa (A) and BHK (B) cells were grown with (control) and without (dyn^K44A for HeLa cells or antisense CHC for BHK cells) tetracycline for 48 hours and with and without 2 mM butyric acid (ba) for the last 24 hours. The cells were then incubated with increasing concentrations of Shiga toxin for 2 hours and protein synthesis measured. The error bars represent deviations between duplicates.