Fig. 1. Non-hydrolyzable analogues of ATP have no effect on CV exocytosis in vitro. CSCs were prepared in IM and were incubated in the absence of nucleotides (open circles) or with either ATP (closed circles), the poorly hydrolyzable analogue ATP--S (open triangles) or the non-hydrolyzable analogue AppNHp (closed triangles) for 90 minutes at room temperature (20°C) with gentle mixing. Ca ²⁺-dependent exocytosis was then measured in the continued presence of the added nucleotides. Mean±s.e.m. are shown, n=3.