Fig. 6. Anti-NSF antibodies do not affect membrane fusion. (A) CSCs were incubated with no antibody (open circles), 50 µg/ml anti-NSF (closed circles) or 50 µg/ml anti-hyalin (open triangles). After 1 hour incubation at room temperature, Ca²⁺ sensitivity of exocytosis was determined. Mean±standard error of the mean are shown, n=3. (B) CVs were incubated with no antibody (open circles), 50 µg/ml anti-NSF (closed circles) or 50 µg/ml anti-hyalin (open triangles). After 1 hour incubation at room temperature Ca²⁺ sensitivity of CV/CV fusion was determined. Mean±s.e.m. are shown, n=3.