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Fig. 5. PPPY/Nedd4.1 and PTAP/Tsg101 interactions take place in different compartments. (A) Gag/CD63 co-staining in 293T cells expressing the Y121D-mutated provirus. (B) Gag/Nedd4.1 co-staining in 293T cells transfected with the wild-type provirus and Nedd4.1-C867S-expressing construct. (C) Gag/CD63 co-staining in 293T cells expressing the P127L-mutated provirus. (D) Gag/CD63 co-staining in 293T cells transfected with the wild-type provirus and Tsg101 siRNAs. Each row shows a median section of cells recorded by confocal microscopy using a 63x objective and a 2x (A) or 4x (B,C,D) zoom. Overlays of red and green pictures are shown as well as bright light pictures. Zoomed images of colocalization areas are also presented (boxed).