Fig. 6. Gag/Nedd4.1 interaction at the plasma membrane precedes Gag/Tsg101 interaction in late endosomes. (A) Gag/Nedd4.1 co-staining in 293T cells co-transfected with wild-type XMT provirus, Tsg101 siRNAs and Nedd4.1-C867S construct. (B) Gag/Nedd4.1 co-staining in 293T cells co-transfected with XMT P127L provirus and Nedd4.1-C867S construct. (C) Gag/CD63 co-staining in 293T cells co-transfected with XMT Y121D provirus and Tsg101 siRNAs. Each row shows a median section of cells recorded by confocal microscopy using a 63x objective and a 4x (A,C) or 2x (B) zoom. Overlays of red and green pictures are shown as well as bright light pictures.