Fig. 4. Immunolocalization of dynamin isoforms in isolated ECL cells using subtype-specific antibodies. Dyn1 immunoreactivity was found in the cytoplasm and at the plasma membrane (A). Dyn2 immunoreactivity was also found in the cytoplasm, the perinuclear region and more strongly at the plasma membrane (B). Dyn3 was mainly detected in the perinuclear area (C). Negative controls (secondary antibodies only) did not produce unspecific staining (D-E'). Bars, 2 µm.