Fig. 8. Rolipram inhibits migration that is associated with dynamic regulation of microspikes. Cells were cultured on laminin coated glass bottomed dishes for 24 hours before being transferred to serum-free media. (A) Carrier (0.1% DMSO) or (B) rolipram (10 µM) was added and cell behaviour observed by timelapse microscopy; images were taken every 5 minutes for 6 hours (see Movies 1, 2, http://jcs.biologists.org/supplemental). (A,B) Stills from those movies, taken at 90-minute intervals. Positions of two cells in each time-series are boxed. Position of a motile peripheral microspike is shown in A (arrows). Scale bar, 40 µm. (C) The range of cell speeds observed under each condition is represented as a box and whisker plot in which the median (solid line across box), mean () and outliers (*) are indicated. Statistical analysis of the two data sets (control, n=31; rolipram, n=28) indicated that the reduced rate of random cell movement in the presence of rolipram was highly significant (Student's t-test, P<0.001).