Fig. 6. Arf6-GDP localizes with EFA6 and ezrin in actin- and PIP₂-enriched structures of the plasma membrane. BHK-21 cells were transfected with Arf6(T44N)-HA alone or with GFP-Rab5, GFP-Rab7, PH-PLC-GFP or PH-EFA6-GFP. After fixation, the cells were probed with anti-HA antibody to detect Arf6, in combination with phalloidin, anti-Giantin, anti-ezrin, anti-Tfn-R or anti-clathrin-heavy-chain antibodies and analysed by xy or xz (for WGA and anti-Giantin labelling) confocal sectioning. The cell surface was stained using Texas-red-conjugated WGA. Arrows indicate some zones of co-staining. Scale bars, 15 µm.