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Fig. 2. Structure and the mutation point of AtMinD1 in the arc11. (A) Chromosomal location of AtMinD1 and domain structure of its product. (B) Sequence alignment of MinD proteins from Arabidopsis (At; database accession number AB030278), Oryza sativa (Os; AP001129), Chlorella vulgaris (Cv; AB001684), Escherichia coli (Ec; J03153) and Pyrococcus furiosus (Pf; NC_003413), with secondary structure elements based on structural (Hayashi et al., 2001; Sakai et al., 2001) and membrane localization analyses (Szeto et al., 2002; Hu and Lutkenhaus, 2003), and the PSIPRED secondary structure prediction program (http://bioinf.cs.ucl.ac.uk/psipred/). A single base substitution of AtMinD1 at position Ala 296 in {alpha}11 helix, changing Ala(GCG) to Gly(GGG), is indicated by arrowheads (A,B) and boxed in (B). (C) Assignment of the AtMinD1 N-terminal region responsible for chloroplast targeting by localization analysis of nonfused and AtMinD1 N-terminus-fused (AtMinD1(1-64)) GFP. CLSM images of GFP (green), chlorophyll autofluorescence (Chl, red) and DIC are shown. Bar, 5 µm.