Fig. 3. Quantification of endogenous and total AtMinD1 transcripts in Arabidopsis plants. Total RNAs from whole seedlings of Arabidopsis WT, arc11 and transgenic arc11 plants (11HA38 and 11HA42 as complemented lines, 11HA2 and 11HA7 as division-inhibited lines, 11HA44 as a partially complemented line, T₄ generation) harboring the AtMinD1-dHA::Tnos transgene were analyzed by TaqMan real-time quantitative RT-PCR system. Primer sets specific to the coding region and 3'-UTR of AtMinD1 were employed to monitor total (white bars) and endogenous (black bars) AtMinD1 transcript levels, respectively. Relative amounts of AtMinD1 transcripts to 18S ribosomal RNA are shown as the means±s.e.m. (with WT=1) from three different plant samples.