Fig. 4. Complementation of the arc11 mutant with appropriate expression of wild-type AtMinD1-dHA. Chloroplasts in leaf petioles of 15-day seedlings were microscopically observed. (A) WT. (B) arc11 mutant. A minichloroplast is indicated by an arrowhead. (C) Complemented arc11 transgenic plant (11HA38, T₄ generation, see Fig. 3). (D) Division-inhibited arc11 transgenic plant (11HA2, T₄ generation, see Fig. 3). (E-H) Partially complemented transgenic arc11 plants containing slightly expanded and surface-rugged chloroplasts compared to WT and complemented plants. (E) WT. (F) Complemented plant (11HA38, identical to (C)). (G) A segregated plant of 11HA38 in the T₂ generation showing a partially complemented phenotype. (H) Partially complemented plant (11HA44, T₄ generation, see Fig. 3). Bars, 10 µm.