Fig. 5. Inhibition of chloroplast division by overexpression of AtMinD1(A296G) in transgenic Arabidopsis. (A) Relative amounts of total (white bars) and endogenous (black bars) AtMinD1 transcripts to 18S ribosomal RNA analyzed by quantitative RT-PCR (see Fig. 3). Data are shown as the means±s.e.m. (with WT=1) from three different plant samples. (B) Images of chloroplasts in leaf petioles of WT and CaMV35S-AtMinD1(A296G) (11S) plants. Some populations of chloroplasts look vacuolated internally (arrowheads, see Fig. 4D). Bar, 10 µm.