Fig. 7. Effect of the A296G mutation on AtMinD1 protein-protein interaction in the yeast two-hybrid system. AH109 cells harboring two expression vectors were grown on selection media plates at 30°C. (A) A 3-day plate lacking Leu and Trp (left) and a 5-day plate lacking His, Leu and Trp (right). (B) Growth of yeast cells on -His plates. Controls and classification of yeast growth are described in Materials and Methods.